Figure 9.

To analyze whether a recovery period from TNFα may have effects on the localization of changed TJ proteins due to treatment with the cytokine, confocal laser scanning immunofluorescence microscopy was performed subsequent to experiments. (A) Claudin-1 (green) and ZO-1 (red), and (B) claudin-3 (green) and occludin (red) were stained. Nuclei were stained in blue (DAPI). The yellow signal in the merged pictures constitutes a colocalization of the stained TJ proteins (scale bar: 20 µm; n = 3; representative pictures).