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. 2021 Aug 16;22(16):8784. doi: 10.3390/ijms22168784

Figure 1.

Figure 1

p53R248Q resulted in regulation of p-AKT. (A) The level of p-AKT was increased after p53R248Q overexpression in OVCAR3 cells. It should be noted that OVCAR3 cells were transiently transfected with either empty plasmid (1 μg/mL), wtp53 (1 μg/mL), or p53R248Q (1 μg/mL) for 24 h and then stimulated with EGF for 15 min. GAPDH expression was measured to serve as a loading control. (B) The band intensity of the immunoblots was quantified using ImageJ software. The relative intensities of p-AKT expression were normalized with the control group. (wtp53: wild-type p53 plasmid; p53R248Q: p53R248Q mutation plasmid).