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Transcripts encoding ASTCRs are expressed in the CG. RT-PCR amplification of full-length open reading frames encoding the four respective H. americanus ASTCRs from pooled CG cDNAs. Expected amplimer sizes: ASTCR1—1263-bp; ASTCR2—1305-bp; ASTCR3—753-bp (arrowhead); and ASTCR4—1032-bp (arrowhead).
