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Insulin signaling protein levels in the aorta following rapamycin treatment. (A) phosphorylated protein kinase B (pAkt (Ser473)) levels relative to total protein kinase B (Akt) levels. (B) Mitogen-activated protein kinase (MAPK) levels were significantly increased due to sex (two-way ANOVA ^ p < 0.05) and due to the rapamycin treatment, particularly in females (post hoc analysis * p < 0.05); n = 3–4/group (Figure S1).
