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Dominant negative p50cdc37 inhibits MAPK activation. Cos-1 cells transiently transfected by using Targefect with pMT2-Raf-1 and p50cdc37 or p50cdc37ΔC, or with vector alone, were split; one set of duplicates was serum starved, while the other was stimulated with EGF. Solubilized extracts were then analyzed either with anti-active-Erk rabbit antiserum (bottom) or for levels of expression with the indicated antibodies (top three panels).
