Table 1.
Estimated costs related to the consumable reagents and materials for the SMA newborn screening first-tier test (all based on real-time PCR), including the consumables for the DNA extraction step that may differ in the commercial and in-house procedures. These costs do not take into account the second-tier assay. The calculations considered single reactions per sample in a 384-well plate and for 33,521 samples per month (see text for details). Note that some laboratories may choose to perform the test in replicates, according to the results obtained in their validation step.
| Real-Time PCR Assay Cost Per Reaction | ||||
|---|---|---|---|---|
| Simplex Test | Multiplex Test | |||
| In-House Standardized | Commercial Tests | In-House Standardized | Commercial Tests | |
| Consumables | USD 1.68 | USD 4.42 | USD 2.04 | USD 5.99–12.76 |
| (R$ 9.64) | (R$ 25.35) | (R$ 11.68) | (R$ 34.40–73.23) | |
USD 1.00 = R$5.74 (14 March 2021).