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. Author manuscript; available in PMC: 2021 Aug 27.
Published in final edited form as: Nature. 2020 May 20;582(7811):283–288. doi: 10.1038/s41586-020-2323-8

Figure 1. pUG tails are added to mRNA fragments in vivo.

Figure 1.

a, Assay to detect gene-specific pUG RNAs. Note: (AC)9 RT oligo can anneal anywhere along the pUG tail. b, oma-1 pUG PCR on total RNA isolated from animals of indicated genotypes, +/− oma-1 dsRNA (RNAi). rde-3 mutants were rescued as described in Main text and Methods. gsa-1, which has an 18nt long genomically encoded pUG repeat in its 3’UTR, is a loading control. Wild-type (WT) vs. rde-3(ne3370) and WT vs. rde-3(ne298) data is representative of >10 and 2 biologically independent experiments, respectively. c, oma-1 pUG PCR on total RNA from animals of indicated genotypes, +/− oma-1 dsRNA. Data is representative of 3 biologically independent experiments. d, Sanger sequencing chromatogram (red=T, black=G, blue=C, green=A) of an oma-1 pUG PCR product.