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. Author manuscript; available in PMC: 2021 Aug 27.
Published in final edited form as: Nature. 2020 May 20;582(7811):283–288. doi: 10.1038/s41586-020-2323-8

Extended Data Fig. 2. RNAi-triggered pUGylation and pUG RNA–directed gene silencing are general and sequence-specific.

Extended Data Fig. 2.

a,gfp::h2b, rde-3(−); gfp::h2b and WT (no gfp::h2b) animals were fed E.coli expressing either empty vector control or gfp dsRNA. b, WT and rde-3(−) animals were fed E.coli expressing empty vector control and either oma-1 or dpy-11 dsRNA. For a and b, gfp, dpy-11 and oma-1 pUG RNAs were detected using the assay outlined in Fig. 1a. Data is representative of 3 biologically independent experiments. c, rde-1(ne219); oma-1(zu405ts) animals were injected with either an oma-1 (n=6) or gfp (n=10) pUG RNA. n=3 for no injection. % embryos hatched was scored for the progeny of injected animals. Inset: injected RNAs run on a 2% agarose gel to assess RNA integrity. Error bars: s.d. of the mean. d, rde-1(ne219); gfp::h2b animals were injected with either an oma-1 or gfp pUG RNA (n=10 for both, 3 for no injection). Mean % progeny with gfp::h2b silenced is indicated ± standard deviation (s.d.). For c and d, all pUG tails were 36nt in length.