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. Author manuscript; available in PMC: 2021 Aug 27.
Published in final edited form as: Nature. 2020 May 20;582(7811):283–288. doi: 10.1038/s41586-020-2323-8

Extended Data Fig. 3. RDE-3–mediated pUGylation is necessary for RNAi.

Extended Data Fig. 3.

a, Animals of the indicated genotypes (all harboring the oma-1(zu405ts) mutation) were treated +/− oma-1 dsRNA. For each experiment, % embryos hatched was scored at 20°C and averaged for 6 individual animals per treatment for each genotype. rde-1(ne219) mutants, which cannot respond to dsRNA3, serve as a control for this experiment. Error bars: s.d. of the mean for 3 biologically independent experiments. b, Control or rde-3(ne298) animals (all rde-1(ne219); oma-1(zu405ts) background) were injected with oma-1 pUG RNAs and % embryos hatched was scored at 20°C. n=10 noninjected and 16 injected animals for control. n=8 noninjected and 14 injected animals for rde-3(ne298). Error bars: s.d. of the mean.