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. Author manuscript; available in PMC: 2021 Aug 27.
Published in final edited form as: Nature. 2020 May 20;582(7811):283–288. doi: 10.1038/s41586-020-2323-8

Extended Data Fig. 4. pUG tails must be appended to sense RNAs of >50nt for functionality.

Extended Data Fig. 4.

rde-1(ne219); oma-1(zu405ts) animals were injected with: a, an oma-1 pUG RNA consisting of the sense or antisense strand of the same 541nt long oma-1 mRNA fragment (beginning at the aug) with a 36nt 3’ pUG tail (n=9 for both; n=3 for no injection). b, oma-1 pUG RNAs consisting of oma-1 mRNA fragments of varying lengths (with position 1 starting at the aug of the oma-1 mRNA sequence) all appended to a 36nt pUG tail. n=6 (no injection), 10 (1–50), 17 (1–100), 8 (1–270), 9 (271–541) and 15 (1–541). For a and b, % embryonic arrest was scored at 20°C. Error bars: s.d. of the mean.