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. 2021 Aug 18;22(16):8894. doi: 10.3390/ijms22168894

Figure 2.

Figure 2

CRISPR/Cas9 strategy to generate Zdhhc19 KO mice. (A) targeting scheme of Zdhhc19 KO mice. Yellow boxes represent exons of the Zdhhc19 gene on mouse chromosome 16, and black arrows (F1, F2 and R1) indicate the site of designed primers for genotyping. Red line indicates the target regions of sgRNAs. Exon 3–6 was deleted upon the injection of the mixtures of Cas9 and Zdhhc19 sgRNA into zygotes. (B) PCR of wildtype (+/+), heterozygous (+/−), and homozygous (−/−). A 536bp band was amplified from the Zdhhc19 KO allele using F1 and R1 primers, and a 617bp band was amplified from the wildtype allele using F1 and R2 primers. (C) Zdhhc19 mRNA expression levels in control and Zdhhc19 KO adult testes were examined by RT-qPCR. Data are presented as mean ± SEM. *** p < 0.001.