Figure 2.

Potential roles of PGK1 in VSMC growth and migration. A10 cells with 24 h siRNA transfection were further used to examine cell viability, and silencing efficiency of PGK1 siRNA (siPGK1) was confirmed by immunoblot (A). An Oris^® cell migration assay kit was used to evaluate cell migration in VSMCs with PGK1 knockdown (B). The influence of silencing PGK1 on distribution of cell-cycle phases was also analyzed using a flow cytometer (C). siNC, the negative control (scramble) siRNA. All siRNAs were applied on VSMCs under culture medium with 10% (v/v) fetal bovine serum (FBS). Photographs of each group were taken at 40× magnifications. Data are representative of at least three independent experiments (n = 6/group). † p < 0.05 and ‡ p < 0.01 compared with the control group (10% FBS + siNC).