Figure 5.

Regulatory effects of hypoxia and growth factors (VEGF-A and PDGF-BB) on PGK1 expression in VSMCs. Cells were treated with 25 ng/mL of recombinant proteins (VEGF-A and PDGF-BB) or with the hypoxic condition for 24 h under low-serum (0.2% FBS) medium (A). Synergistic effects of hypoxia and PDGF-BB on PGK1 expression were investigated under the low-serum condition (B). Investigation of the role of VEGF receptor-1 (VEGFR1) on PDGF-BB-mediated PGK1 up-regulation (C). siPDGFRB, PDGFRB siRNA; siNC, negative control siRNA. Data are representative of three independent experiments. The significance level is denoted with * for p < 0.05 and ** for p < 0.01.