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. 2021 Aug 17;22(16):8822. doi: 10.3390/ijms22168822

Figure 6.

Figure 6

Role of HIF-1α in PDGF-BB-mediated PGK1 expression in VSMCs. Cells were pre-transfected with siRNAs for 48 h and then treated with 25 ng/mL PDGF-BB for 20 min or 24 h. Confirmation of the relationship between PDGF receptor-β (PDGFRB)-mediated signaling cascade and PGK1 expression. Cells were pretreated with 30 μM LY294002, a PI3K inhibitor, for 30 min (or pretreated with HIF-1 siRNA for 48 h) and then co-treated with 25 ng/mL PDGF-BB for 20 min or 24 h. siPDGFRB, PDGFRB siRNA; siNC, negative control siRNA. Data are representative of three independent experiments. The significance level is denoted with ** for p < 0.01.