Figure 3.

Antibacterial dose-response curves. The parental and hybrid peptides were tested against E. coli strains D31 (A) and JM83 (B) across the concentration range 0.015–250 µM. Concentration-response curves were generated using decreasing peptide doses. The y-axis shows the optical density at 600 nm (OD₆₀₀) and the x-axis shows the assay time from 0 to 16 h. Each panel shows the total growth inhibition peptide concentrations (red line), different concentrations (gray lines) and the normal bacterial growth curve in the absence of AMPs (black line).