Figure 3.

Decellularized muscle matrix (DMM) had no differences in collagen staining with old age. (A) Hematoxylin and Eosin (H&E) staining confirmed the absence of cell nuclei at all ages, indicating successful decellularization. Masson’s trichrome did not show age differences, while Picrosirius Red staining shows a thickening of the collagen with age in DMM. (B) There is no difference in porosity with age as measured with H&E staining. The amount of collagen area stained with Pricoririus Red staining is more significant in immature muscle compared to young adult. Although the mean area in old age is higher than in young adults, it is not statistically higher nor different from the immature muscle. Scale bar = 100 µm. Groups that do not share a letter (e.g., A or B) are statistically different according to a one-way ANOVA followed by a two-tailed Tukey’s correction (p < 0.05, n = 6).