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. 2021 Aug 19;22(16):8931. doi: 10.3390/ijms22168931

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of antibody dilution on the binding of monoclonal and polyclonal anti-Z-DNA antibodies to natural DNA. The binding of various dilutions of monoclonal and polyclonal anti-Z-DNA antibodies to DNA coated at a concentration of 2.5 μg/mL was assessed by ELISA. (A) Results for the monoclonal antibody; (B) results for the polyclonal antibody. Results are reported in terms of OD 450 nm values. All treatments were performed in duplicate. The means and standard deviations are presented.