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. 2021 Aug 19;22(16):8931. doi: 10.3390/ijms22168931

Figure 3.

Figure 3

The effects of soluble Br-poly(dG-dC) on the binding of polyclonal anti-Z-DNA antibodies to MC DNA. To show the specificity of the polyclonal anti-Z-DNA antibodies for Z-DNA in MC DNA, the ability of soluble Br-poly(dG-dC) to inhibit antibody binding was tested (A). Br-poly(dG-dC) was used a source of Z-DNA; unbrominated poly(dG-dC) was the control (B). The binding of the antibody preparations at dilutions of both 1:1000 and 1:2000 was tested. The results are presented as OD 450 nm values. All treatments were performed in duplicate. Means and standard deviations are shown.