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. 2021 Aug 19;22(16):8931. doi: 10.3390/ijms22168931

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Time course of antibody binding to natural DNA and Br-poly(dG-dC). The binding of monoclonal and polyclonal anti-Z-DNA antibodies to natural DNA (MC and EC) and Br-poly(dG-dC) was tested at 5, 20 and 60 min. (A,C,E,G) Results of monoclonal anti-Z-DNA binding; (B,D,F,H) polyclonal anti-Z-DNA binding. DNA was coated to plates at 2 μg/mL. Antibody preparations were titered, with the starting concentrations determined by a prior experiment to determine the dilutions covering the linear portion of antibody binding to DNA. Results are presented as the average OD 450 nm values of duplicate wells. Means and standard deviations are shown.