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. 2021 Aug 18;22(16):8901. doi: 10.3390/ijms22168901

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Flow cytometry revealed high-level expressions of beta 2-microglobulin and human leukocyte antigen A2 in three prostate cancer stem cell populations. (A–D) Analyses showed over 98% beta 2-microglobulin (B2M)⁺ cells in prostate cancer stem cells (PCSCs) (left panel, blue graphs), similar to interferon gamma (IFNγ) pre-treated cells (green graphs). The human leukocyte antigen A2 (HLA-A2) staining (right panels) in PIX, PXI and PXIV PCSCs revealed more than 99% positive cells (A,B,D). The PCSC population PXIII displayed a maximum of 2% HLA-A2⁺ cells (C, right panel) when untreated (blue graph), and only 0.9% after treatment with IFNγ (green graph). For all the analyses, isotype controls are displayed as red graphs.