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. 2021 Aug 18;22(16):8877. doi: 10.3390/ijms22168877

Figure 4.

Figure 4

RT-qPCR was performed to validate the effective knockdown of the CREB, AP-1 and NF-κB1 genes of HUVECs by the transfection of pooled siRNAs for CREB, AP-1, and NF-κB1 from 10 nM to 40 nM, followed by 30 µg ADSC-LPS-exo treatment for 24 h. The HUVECS treated with ADSC-LPS-exo alone were used as controls. The HUVECs treated with scramble siRNA first, followed by ADSC-LPS-exo treatment were used as mock control. n = 6 for each condition. (*, a p-value < 0.05 with at least 2-fold reduced expression of the gene).