Figure 2.

Time-dependent gene expression for rat heat shock protein-70 (Hspa4), heme oxygenase-1 (Hmox1), and metallothionein (Mt1) (A) and for GCLC (Gclc) and GCLM (Gclm) (B) in choroid plexus epithelial cells (CPECs) exposed to 500 nM CdCl₂. Non-Zinc supplemented CPECs were pre-treated (12 h) in serum-free medium (SFM) and then divided into two sets: one set was incubated for 12 h in SFM without CdCl₂ (Control), while the other was exposed for 12 h to 500 nM CdCl₂ in SFM (Cd). All treatments were performed at 37 °C. At 3, 6, 9, and 12 h, representative cadmium-exposed and time-matched control cells were collected for analysis of mRNA expression by quantitative real-time polymerase chain reaction (qRT-PCR); mRNA expression for each test gene was normalized to mRNA for rat β-actin (Actb) and GAPDH (Gapdh). Fold-induction was calculated as the ratio of normalized gene expression in Cd-exposed cells to that in time-matched controls. mRNA was analyzed in triplicate in five separate culture preparations. Data are means ± SE; n = 5; * p < 0.05 vs. time-matched Control.