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. 2021 Aug 19;22(16):8923. doi: 10.3390/ijms22168923

Figure 4.

Figure 4

Identification of transferrin receptor as molecular target of aptamer HG1-9. (A) Flow cytometry analysis of HG1-9 binding to Jurkat cells pretreated by different concentrations of trypsin or proteinase K. (B) Flow cytometry analysis of HeLa and Jurkat cells dual-stained by aptamer HG1-9 (labeled by Cy5) and antibody anti-TfR (labeled by PE). The cross-quadrant gate in each bivariate histogram was respectively set according to negative control in different cell lines. (C) Confocal imaging of HeLa cells dual-stained by aptamer HG1-9 (labeled by FITC, 200 nM) and anti-TfR antibody (labeled by PE-second antibody, dilution of 1:20), the scale is 10 μm. (D) Relative median fluorescence intensities of aptamer HG1-9 and anti-TfR antibody binding to HeLa cells after siRNA knockdown. Relative median fluorescence intensities were acquired according to formula of F = (F1 − F0)/F0. Blank bars represent cells without any treatment, grey bars represent cells treated by si-NC sequence, and white bars represent cells treated by si-TfR. The ctr sq and IgG were as the negative control, t-test, n = 3, * p < 0.05; ** p < 0.01.