Figure 4.

Aptamer-mediated NoV pull-down from stool suspension using different aptamers. The Ct values are shown on the y-axis, and each bar on the x-axis represents the aptamer used during the pull-down. Statistically significant differences compared to the negative control (NC) are indicated with asterisks above each bar. The p-value indication for the asterisks is: not significant (ns) for p-value > 0.05, two asterisk (**) for p-values between of 0.0021–0.0332 and four asterisks (****) for p-values < 0.0001. The aptamer mediated pull-downs were completed for each aptamer in triplicate from a 100-fold dilution from partially purified stool (A) and 1000-fold dilution of the same preparation (B). In (B) no Ct values were obtained for NC and AG3. Therefore, the maximum number of cycles run in the PCR program (45) is shown.