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Susceptibility of A. hydrophila and A. salmonicida to three sequential extracts treatment determined by disk diffusion assay. Disks loaded in duplicates with 50 µL of (a) AWME1 (Ex#1) and AWME2 (Ex.#2); (b) AWME3 (Ex.#3) and a mixture of the three SEs (Mix) at concentration 40 mg/mL; (c) Positive control (Doxycycline, 100 µg/mL) and the extraction reagent as a negative control (N.ct).
