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. 2021 Aug 17;22(16):8829. doi: 10.3390/ijms22168829

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Susceptibility of (a) A. hydrophila and (b) A. salmonicida bacteria strains to various SEs measured by Disk diffusion assay. The bacteria subjected to AWME1 (Ex#1), AWME2 (Ex#2), and AWME3 (Ex#3), respectively, and a mixture of three extract (Mix) adjusted to the same 40 mg/mL concentration. The IZD values calculated after 12 h and 24 h of incubation at 26 °C by measuring the diameter of inhibition zones (IZD) surrounding of the discs (in mm). Doxycycline (DOX) 100 µg/mL used as a recommended antibacterial positive control. All values are represented as mean ± SD, in triplicate (n = 3). All results analyzed by two-way ANOVA, followed by Tukey Multiple Comparisons Test. Data represented as a significant difference when it was compared with the positive control, and p-Value was ranged between ** p = 0.0047 and **** p < 0.0001.