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. 2021 Aug 17;22(16):8829. doi: 10.3390/ijms22168829

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Susceptibility of A. hydrophila (a) and A. salmonicida (b) determined by disk diffusion assay of AWME3 (the third sequential extract) of BSFL fat. The bacteria strains were subjected to concentrations of 1.88, 3.75, 7.5, 15, 30, and 40 mg/mL of AWME3 from BSFL fat. The IZD were measured after 12 h and 24 h of incubation at 26 °C by measuring IZD surrounded of the disks. Doxycycline (DOX) used as the recommended positive antibacterial control. All values are represented as mean ± SD of three (n = 3) independent experiments. Data were analyzed by two-way ANOVA, followed by Tukey Multiple Comparisons Test. Data represented as significant difference as compared to positive control, and p-Value was ranged between ** p = 0.0019 and **** p < 0.0001.