Figure 3.

Aze inhibited the LPS-induced phosphorylation of JNK and c-Jun in BV2 cells. Cells were co-treated with LPS (1 μg/mL) and SP600125 (10 μM) or the indicated concentrations of Aze for 24 h. Control cells were treated with vehicle only. Cell lysates were then prepared and analyzed by western blotting using anti-phospho-JNK (p-JNK) and JNK3 antibodies (A) or anti-phospho-c-Jun (p-c-Jun) and c-Jun antibodies (B). β-Actin was used as a loading control. Representative blots are shown. Data are presented as the mean ± SEM from three independent experiments. # p < 0.05 and * p < 0.05 vs. vehicle-treated control and LPS-treated cells, respectively.