Figure A1.

The morphology of the diploid mutant embryos and one-day-old larvae. (A) The morphology of the diploid mutant embryos. The eggs of the mock-treated groups were injected with sgRNA. The eggs of the treatment groups were injected with a mixture of sgRNA and Cas9 nucleases. At 24 h AEL, there was no difference in development between the embryos of the mock-treated group and the treatment group. However, obvious immunization responses occurred in the embryos of treatment groups. At 48 h AEL, the treatment group’s embryos were still at stage six, while the mock-treated embryos were at stage seven. At 72 h AEL, the embryos of the treatment group were at stage eight, while the embryos of the mock-treated group were at stage nine. Scale bars, 200 µm. (B) The morphology of the epiprocts of the one-day-old diploid mutant larva. The larva on the left was a genetic male; the larvae in the middle and on the right were genetic females. Scale bars, 200 µm.