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. 2021 Aug 21;22(16):9040. doi: 10.3390/ijms22169040

Figure 2.

Figure 2

Co-overexpression of OsNRT2.1 and OsNAR2.1 increased nitrogen use efficiency in transgenic rice plants. qRT-PCR analysis showed the expression of (a) OsNAR2.1 and (b) OsNRT2.1 of WT, p35S:OsNAR2.1 transgenic lines (NAR1, NAR2), p35S:OsNRT2.1 transgenic lines (NRT1, NRT2), and p35S:OsNAR2.1-p35S:OsNRT2.1 transgenic lines (NN1, NN2). RNA was extracted from root. Error bars: SE (n = 3). (c,d) 15N influx rates of transgenic lines. WT and transgenic seedlings were grown in 1 mM NH4+ for 3 weeks and nitrogen starved for 1 week. 15N influx rates were then measured at (c) 0.5 mM 15NO3−, (d) 0.5 mM 15NH4+ during 5 min. DW, dry weight. (e) Photograph of WT and transgenic lines in the field experiment. Comparison of (f) agronomic nitrogen use efficiency and (g) nitrogen recovery efficiency between the NP and transgenic lines. Error bars: SE (n = 4). The different letters indicate a significant difference between the transgenic line and the WT (p < 0.05, one-way ANOVA).