Skip to main content
. 2021 Aug 21;22(16):9035. doi: 10.3390/ijms22169035

Figure 2.

Figure 2

Heme affinity-purified proteins from total extracts of SARS-CoV-2 infected Vero cells and identification by mass spectrometry. SDS-PAGE resolved and Coomassie blue stained proteins: (a) whole virus-infected Vero cells extract and (b) eluate from a hemin-agarose column purification (after albumin depletion). Bands excised for protein identification by LC–MS/MS are indicated by black boxes 1–8. The proteins identified were N (P0DTC9), M (P0DTC5), S (P0DTC2), and replicase polyprotein 1a/1ab (P0DTC1/P0DTD1). The peptides in the replicase polyprotein 1a/1ab were identified as Nsp2, Nsp3, and Nsp7. The identified proteins in each band are shown as 3D structures retrieved from the I-TASSER site (https://zhanglab.ccmb.med.umich.edu/I-TASSER/, accessed on 20 September 2020). (c) Western blot revealed with COVID-19 patient convalescence sera pool (n = 5); arrowheads indicate two bands showed in the eluate.