FIG. 5.

Influence of Ral-GEF activity on fos gene regulation in PC12 cells. Cells were transfected with a Fos-luciferase reporter construct along with a minimal thymidine kinase promoter-Renilla reporter construct (as an internal control for transfection efficiency) and various expression plasmids. In some instances, NGF (50 ng/ml) was added to the cells 24 h later. After 2 days, firefly luciferase and Renilla luciferase activities were measured in cell lysates. The data are reported as the ratio of firefly-luciferase to Renilla luciferase activities and represents the average (± standard deviation) of at least two experiments, each performed in duplicate.