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. 2021 Aug 20;22(16):8998. doi: 10.3390/ijms22168998

Figure 4.

Figure 4

Atomic force microscopy reveals Pum2 binding to eIF4E mRNA. (A) Coomassie-stained SDS-gel upon Nickel-NTA purification of His6-tagged full length Pum2. (B) Relative enrichment of eIF4E and Slc32a1 mRNA in His6-tagged full length Pum2 pulldown. (C,D) Scheme for AFM experimental setup (left), representative AFM images (middle), and height profiles (right) for Pum2 particles bound to immobilized eIF4E 3′-UTR RNA probes. See text for explanations. P-values were calculated using one-sample t-test within samples and unpaired Students t-test between samples. * p < 0.05, ** p < 0.01, 4 biological replicates.