Table 2. Hematology, clinical biochemistry, and serum IgE and histamine levels in male Sprague-Dawley (SD) rats.
| Category | Male | ||||||
|---|---|---|---|---|---|---|---|
| G1 | G2 | G3 | G4 | G5 | G6 | ||
| Hematological findings | RBC (10 6 cells/μL) | 7.37±0.13 | 7.38±0.14 | 7.31±0.11 | 7.48±0.14 | 7.50±0.10 | 7.37±0.14 |
| HGB (g/dL) | 14.52±0.20 | 14.57±0.22 | 14.57±0.31 | 14.58±0.23 | 14.7±0.12 | 14.37±0.24 | |
| HCT (%) | 46.30±0.40 | 46.15±0.68 | 45.87±0.85 | 46.22±0.57 | 46.42±0.36 | 45.67±0.89 | |
| MCV (fL) | 62.95±0.81 | 62.57±0.55 | 62.73±0.56 | 61.82±0.57 | 61.95±1.00 | 62.02±0.09 | |
| MCH (pg) | 19.72±0.27 | 19.73±0.16 | 19.90±0.17 | 19.50±0.31 | 19.57±0.27 | 19.50±0.13 | |
| MCHC (g/dL) | 31.33±0.19 | 31.60±0.07 | 31.72±0.24 | 31.55±0.23 | 31.62±0.12 | 31.48±0.19 | |
| RETIC (10 9 cells/μL) | 218.7±8.4 | 213.2±9.4 | 225.7±11.4 | 238.7±4.2 | 198.7±11.3 | 222.8±10.0 | |
| PLT (10 3 cells/μL) | 1156±63 | 1168±55 | 1161±66 | 1210±39 | 1228±28 | 1170±47 | |
| WBC (10 3 cells/μL) | 10.81±1.14 | 10.90±0.81 | 9.68±0.95 | 8.56±0.44 | 7.15±0.76 | 8.14±0.87 | |
| Clinical biochemical findings | TP (g/dL) | 5.90±0.06 | 6.07±0.08 | 5.97±0.11 | 6.00±0.09 | 6.10±0.09 | 6.13±0.10 |
| ALB (g/dL) | 3.85±0.04 | 3.88±0.03 | 3.83±0.06 | 3.85±0.03 | 3.97±0.04 | 3.88±0.06 | |
| A/G ratio | 1.88±0.04 | 1.78±0.03 | 1.78±0.04 | 1.80±0.04 | 1.87±0.04 | 1.73±0.02* | |
| T-BIL (mg/dL) | 0.04±0.00 | 0.05±0.00 | 0.04±0.00 | 0.03±0.00 | 0.03±0.00 | 0.05±0.00 | |
| ALP (U/L) | 596.3±33.6 | 711.0±57.2 | 532.3±38.5 | 577.2±30.6 | 560.2±35.6 | 647.5±47.3 | |
| AST (U/L) | 90.33±6.35 | 119.2±30.2 | 92.67±4.34 | 81.00±4.46 | 106.5±4.8 | 101.5±9.7 | |
| ALT (U/L) | 28.33±3.46 | 45.50±16.37 | 27.17±2.85 | 22.83±1.56 | 29.00±2.66 | 26.17±2.44 | |
| CREA (mg/dL) | 0.44±0.01 | 0.45±0.02 | 0.46±0.02 | 0.44±0.02 | 0.40±0.01 | 0.42±0.01 | |
| BUN (mg/dL) | 11.78±0.60 | 12.60±1.00 | 11.93±0.54 | 10.42±0.74 | 11.17±0.48 | 10.90±0.41 | |
| T-CHO (mg/dL) | 71.00±3.39 | 78.50±4.65 | 76.33±4.26 | 72.33±5.33 | 65.83±5.68 | 82.00±3.01 | |
| TG (mg/dL) | 80.33±7.06 | 99.67±13.84 | 112.5±15.3 | 96.83±13.26 | 81.33±9.54 | 93.67±17.89 | |
| GLU (mg/dL) | 127.7±2.9 | 111.0±4.2 | 103.5±3.7* | 117.0±7.9 | 99.33±3.33* | 99.50±6.82* | |
| CA (mg/dL) | 10.15±0.11 | 10.23±0.05 | 10.13±0.10 | 10.32±0.06 | 10.00±0.10 | 10.23±0.14 | |
| IP (mg/dL) | 8.90±0.18 | 8.68±0.17 | 8.63±0.15 | 8.78±0.13 | 9.02±0.09 | 8.90±0.11 | |
| GGT (IU/L) | 0.39±0.16 | 0.34±0.18 | 0.69±0.26 | 0.34±0.16 | 0.19±0.09 | 0.48±0.26 | |
| CK (U/L) | 529.7±45.1 | 529.7±29.3 | 578.5±60.3 | 452.5±39.0 | 745.5±50.4 | 652.3±79.4 | |
| Na (mmol/L) | 143.0±0.3 | 144.4±0.5 | 143.6±0.3 | 145.2±0.4** | 144.3±0.4 | 144.9±0.4* | |
| K (mmol/L) | 4.68±0.08 | 4.68±0.06 | 4.76±0.08 | 4.87±0.12 | 4.95±0.05 | 4.89±0.10 | |
| Cl (mmol/L) | 101.4±0.3 | 102.8±0.5 | 101.4±0.8 | 103.1±0.5 | 101.5±0.6 | 102.3±0.6 | |
| ELISA analysis | IgE (ng/mL) | 0.233±0.014 | 0.270±0.001 | 0.246±0.015 | 0.181±0.008 | 0.200±0.016 | 0.188±0.004 |
| Histamine (ng/mL) | 0.480±0.040 | 0.383±0.038 | 0.402±0.034 | 0.423±0.026 | 0.519±0.052 | 0.390±0.040 | |
Data are presented as mean ± SEM.
All data were analyzed using Kruskal-Wallis test and the significance of intergroup difference between the control and administered groups was assessed using Dunn’s Rank Sum test.
*vs. Control group: p < 0.05,
** vs. Control group: p < 0.01