Table 3. Hematology, clinical biochemistry, and serum IgE and histamine levels in female Sprague-Dawley (SD) rats.
| Category | Female | ||||||
|---|---|---|---|---|---|---|---|
| G1 | G2 | G3 | G4 | G5 | G6 | ||
| Hematological findings | RBC (10 6 cells/μL) | 7.14±0.20 | 6.72±0.17 | 7.06±0.08 | 7.30±0.09 | 7.47±0.22 | 7.30±0.13 |
| HGB (g/dL) | 14.08±0.30 | 13.78±0.31 | 13.96±0.20 | 14.58±0.12 | 14.74±0.28 | 14.52±0.28 | |
| HCT (%) | 42.54±0.88 | 42.08±1.01 | 42.06±0.46 | 44.42±0.16 | 44.60±0.88 | 44.40±0.76 | |
| MCV (fL) | 59.70±0.89 | 62.70±1.13 | 59.60±0.91 | 60.92±0.69 | 59.84±0.92 | 60.84±0.60 | |
| MCH (pg) | 19.76±0.22 | 20.46±0.06 | 19.78±0.36 | 20.00±0.26 | 19.76±0.29 | 19.88±0.19 | |
| MCHC (g/dL) | 33.12±0.27 | 32.70±0.50 | 33.20±0.15 | 32.84±0.17 | 33.00±0.28 | 32.70±0.15 | |
| RETIC (10 9 cells/μL) | 156.9±10.2 | 170.9±16.1 | 151.9±14.7 | 169.0±5.2 | 143.1±13.7 | 159.8±7.6 | |
| PLT (10 3 cells/μL) | 995.6±167.9 | 858.2±201.0 | 1208±111 | 1164±78 | 1097±56 | 1217±63 | |
| WBC (10 3 cells/μL) | 3.48±0.43 | 4.24±0.97 | 5.62±1.00 | 5.88±1.13 | 4.71±0.35 | 4.38±0.37 | |
| Clinical biochemical findings | TP (g/dL) | 6.10±0.05 | 6.06±0.14 | 6.10±0.23 | 6.04±0.04 | 6.02±0.09 | 6.20±0.13 |
| ALB (g/dL) | 4.14±0.06 | 4.02±0.12 | 3.94±0.19 | 3.90±0.06 | 3.90±0.03 | 4.04±0.09 | |
| A/G ratio | 2.10±0.05 | 1.98±0.08 | 1.84±0.11 | 1.84±0.06 | 1.86±0.04 | 1.88±0.04 | |
| T-BIL (mg/dL) | 0.07±0.00 | 0.03±0.00* | 0.05±0.01 | 0.03±0.01* | 0.04±0.01 | 0.04±0.01 | |
| ALP (U/L) | 354.8±16.1 | 483.2±45.1 | 448.2±37.2 | 433.0±49.5 | 442.4±44.8 | 474.6±48.6 | |
| AST (U/L) | 83.00±2.51 | 96.60±6.17 | 92.60±13.1 | 92.00±2.00 | 82.00±4.32 | 84.20±7.04 | |
| ALT (U/L) | 26.20±0.80 | 27.00±2.55 | 31.20±5.00 | 25.40±2.38 | 33.20±2.06 | 29.60±1.96 | |
| CREA (mg/dL) | 0.43±0.02 | 0.46±0.03 | 0.43±0.01 | 0.41±0.02 | 0.39±0.01 | 0.43±0.02 | |
| BUN (mg/dL) | 19.88±2.57 | 15.58±1.01 | 15.94±0.88 | 13.46±1.22 | 14.62±0.86 | 16.86±1.08 | |
| T-CHO (mg/dL) | 86.80±9.28 | 85.80±3.81 | 87.00±4.55 | 86.60±6.59 | 86.40±3.82 | 97.80±9.41 | |
| TG (mg/dL) | 76.20±19.14 | 47.60±7.47 | 48.60±3.66 | 47.60±16.46 | 52.40±6.54 | 63.80±7.65 | |
| GLU (mg/dL) | 114.0±4.4 | 124.0±6.5 | 104.0±2.7 | 103.6±5.2 | 113.2±4.6 | 120.4±6.6 | |
| CA (mg/dL) | 10.04±0.10 | 9.96±0.13 | 10.16±0.20 | 10.08±0.14 | 10.28±0.04 | 10.44±0.10 | |
| IP (mg/dL) | 7.36±0.25 | 7.30±0.23 | 7.48±0.23 | 7.50±0.22 | 7.76±0.17 | 8.00±0.13 | |
| GGT (IU/L) | 0.34±0.09 | 0.17±0.07 | 0.21±0.13 | 0.32±0.14 | 0.20±0.13 | 0.43±0.12 | |
| CK (U/L) | 606.4±15.2 | 683.2±67.8 | 533.6±118.0 | 491.4±28.3 | 431.6±45.0 | 508.6±114.3 | |
| Na (mmol/L) | 140.8±0.3 | 141.6±0.5 | 141.7±0.7 | 141.9±0.3 | 141.1±0.3 | 141.2±0.3 | |
| K (mmol/L) | 4.44±0.07 | 4.29±0.14 | 4.50±0.16 | 4.44±0.09 | 4.54±0.12 | 4.43±0.22 | |
| Cl (mmol/L) | 100.2±0.3 | 102.5±0.4* | 103.0±0.6** | 101.6±0.9 | 102.3±0.4 | 101.5±0.3 | |
| ELISA analysis | IgE (ng/mL) | 0.222±0.023 | 0.211±0.008 | 0.247±0.035 | 0.211±0.025 | 0.187±0.022 | 0.183±0.016 |
| Histamine (ng/mL) | 0.253±0.030 | 0.311±0.043 | 0.385±0.027 | 0.257±0.034 | 0.570±0.128* | 0.510±0.063* | |
Data are presented as mean ± SEM.
All data were analyzed using Kruskal-Wallis test and the significance of intergroup difference between the control and administered groups was assessed using Dunn’s Rank Sum test.
*vs. Control group: p < 0.05,
** vs. Control group: p < 0.01