Table 1. X and autosomal count ratios detected per gene for every cell type.

These ratios are of raw counts from X and autosomes, not log transformed. The ratio of X/autosome expression is close to or greater than 1 in hub, cyst, GSC, early spermatogonia and late spermatogonia, indicating the presence of dosage compensation. Late spermatogonia may also be interpreted as no DC if they contain most transcripts generated from early spermatogonia. This ratio decreases in meiotic and post-meiotic cells, indicating the loss of dosage compensation. P values are for a two-sided Wilcoxon test with a null hypothesis that X and autosome genes are similarly expressed, interpreted as dosage compensation. Meiotic and post-meiotic cells show an imbalance in x-autosome ratios, indicative of absent dosage compensation, but in pre-meiotic and somatic cells, x and autosomal ratios are near or over 1. The X/autosome ratio is highest in GSC/early spermatogonia, with a statistical enrichment of X chromosome gene expression, interpreted as possible excess dosage compensation in this cell type. DC means dosage compensation.

Cell type	X/autosome ratio of raw counts	Raw p value	Adjusted P value	Interpretation
Hub cells	1.00	1.43e-01	2.86e-01	DC
Cyst cells	1.17	2.34e-02	8.24e-02	DC
Epithelial cells	0.92	1.63e-01	2.86e-01	DC
GSC, Early spermatogonia	1.83	6.44e-05	3.86e-04	Excess DC
Late spermatogonia	0.85	2.06e-02	8.24e-02	DC
Early spermatocytes	0.66	5.35e-08	4.82e-07	No DC
Late spermatocytes	0.63	6.69e-08	5.35e-07	No DC
Early spermatids	0.70	1.65e-05	1.16e-04	No DC
Late spermatids	0.77	1.30e-04	6.50e-04	No DC