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. 2021 Aug 11;19(8):e3001367. doi: 10.1371/journal.pbio.3001367

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© 2021 Velarde et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — (A–F and I–N) Projections of confocal images of third instar eye discs stained with anti-Elav (blue) and anti-Repo (white). (A–F) Effects caused by the down-regulation of Dpp and Hh signalling in the damaged region. The schematic illustration on the left represents a transverse section of an eye disc where the region marked in red (expression domain of GMR-Gal4) corresponds to the area of the discs that has been damaged at the same time that Dpp and/or Hh signalling were depleted. (A) UAS-rpr/+; GMR-Gal4 tub-Gal80^ts/+ damaged eye disc. (B) Damaged UAS-rpr/+; GMR-Gal4 tub-Gal80^ts/ UAS-hh^RNAi; UAS-dpp^RNA33i. (C) Damaged UAS-rpr/+; GMR-Gal4 tub-Gal80^ts; UAS-dpp^RNAi33 (BDSC33618). (D) UAS-rpr/+; GMR-Gal4 tub-Gal80^ts/ UAS-hh^RNAi damaged discs. (E) Control undamaged GMR-Gal4 tub-Gal80^ts/+ disc. (F) GMR-Gal4 tub-Gal80^ts/ UAS-hh^RNAi; UAS-dpp^RNAi33 eye discs. (G and H) Graphs show glial cell density (G) and percentage of mitotic glia cells of discs shown in A–F. (I–N) Effects of the down-regulation of Dpp and Hh signalling in glial cells in damaged discs. In the schematic illustration on the left is indicated in red the region that has been damaged (GMR-QF; QUAS-rpr) and in light blue glial cells. (I) GMR-QF; tub-Gal80^ts; repo-Gal4 QUAS-rpr eye disc. (J) GMR-QF; tub-Gal80^ts/UAS-ptc; repo-Gal4 QUAS-rpr. (K) GMR-QF; tub-Gal80^ts; repo-Gal4 QUAS-rpr/UAS-brk. (L) GMR-QF; tub-Gal80^ts/UAS-ptc; repo-Gal4 QUAS-rpr/UAS-brk. (M) Control undamaged tub-Gal80^ts; repo-Gal4 disc. (N) tub-Gal80^ts/UAS-ptc; repo-Gal4 /UAS-brk. (O and P) Graphs show glial cell density (O) and percentage of mitotic glial cells (P) of discs shown in I–N. Scale bars, 50 μm. Statistical analysis is shown in Table G in S1 Text. The numerical data used in this figure are included in S1 Data. GMR, glass multiple reporter; Dpp, Decapentaplegic; Hh, Hedgehog; RNAi, RNA interference; rpr, reaper.