Fig 9. Overexpression of ihog and tkv^QD in glial cells induces over-migration and proliferation.

(A–F) Third instar eye discs stained with anti-PH3 (red) and anti-Repo (green). Basal layers of the eye disc epithelium. (A) Control tub-Gal80^ts repo-Gal4 eye disc. (B)tub-Gal80^ts/UAS-ihog; repo-Gal4/+. (C) tub-Gal80^ts/+; repo-Gal4/UAS-tkv^QD. (D) tub-Gal80^ts/UAS-tkv^QD/UAS-ci^m1-3; repo-Gal4/UAStkv^QD. (E) tubGal80^ts/UAS-ihog;repo-Gal4/UAS-tkv^QD. (F) hepr75; tub-Gal80^ts/UAS-ihog; repo-Gal4/UAS-tkv^QD. The overexpression of tkv^QD under the control of repo-Gal4 increases the number of glial cells (C). The co-overexpression of UAS-tkv^QD and UAS-ihog under repo-Gal4 increases the density and proliferation of subretinal glial cells and promotes the over-migration of these cells (E). The over-migration phenotype and the increase of glial density caused by the overexpression of UAS-tkv^QD and UAS-ihog with repo-Gal4 was suppressed in the hep^r75 mutant discs. Nonetheless, the glial cell division was not significantly altered (F and H). (G) The graph represents the glial density of discs shown in A-F. (H) Histogram showing the percentage of glial cells in mitosis (Ph3 positive) of discs indicated in A–F. (I) The graph represents the percentage of discs with glial over-migration shown in A–F. Scale bars, 50 μm. Statistical analysis is shown in Table H in S1 Text. The numerical data used in this figure are included in S1 Data.