FIG. 8.

Stable and transient expression of p53 proteins in p53-null MEFs. (A) Western blot analysis of MEFs stably expressing either a vector control (pCB6+) or p53ΔII with or without treatment with 5 nM actinomycin (ActD). (B) Western blot analysis of MEFs transiently expressing a vector control (pCB6+) or the indicated p53 proteins without DNA damage or following treatment with actinomycin D (Act; 5 nM) or UV irradiation (50 J/m²). (C) Western blot analysis of MEFs transiently expressing a vector control (pCB6+), wild-type p53, or p53N-term without DNA damage or following treatment with actinomycin D (ActD; 5 nM) or UV irradiation (50 J/m²).