Table 1.

Identification results of T. benhamiae complex species using MALDI-ToF mass spectroscopy. An identification was deemed valid when log scores were ≥1.7 and at least three out of the four replicates were identified as the same species, strains for which any of these criteria were not met were counted in the column ‘no identification’. When an identification did not match the identity based on ITS+BT genetical analysis, the identification was deemed an ‘incorrect identification.’ For certain clades (in bold) of closely related species/variants combined scores were calculated where identification was considered correct if the strain was identified as either one of the species/variants comprised in the clade.

Clade/Species	Number of Samples
	Total	Correct Identification	Incorrect Identification	No Identification
T. europaeum -japonicum clade
T. europaeum	24	12 (50%)	6 (1 T. benhamiae, 1 T. erinacei, 4 T. japonicum)	6
T. japonicum	10	7 (70%)	2 (1. T. benhamiae, 1 T. erinacei)	1
Combined	34	28 (82%)	4 (2 T. benhamiae, 2 T. erinacei)	2
T. benhamiae clade
T. benhamiae var. benhamiae (white phenotype)	35	26 (74%)	7 (5 T. benhamiae var. luteum, 1 T. africanum, 1 T. concentricum)	2
T. benhamiae var. luteum (yellow phenotype)	16	13 (81%)	2 (2 T. benhamiae)	1
Combined	51	48 (94%)	2 (1 T. africanum, 1 T. concentricum)	1
T. africanum	7	6 (86%)	1 (1 T. benhamiae)	0
T. bullosum	2	2 (100%)	0	0
T. concentricum	43	42 (98%)	0	1
T. verrucosum	7	6 (86%)	0	1
T. erinacei clade
T. erinacei	17	11 (64%)	4 (4 T. cf. erinacei)	2
T. cf. erinacei	2	2 (100%)	0	0
Combined	19	18 (95%)	0	1