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. 2021 Jul 27;8(8):87. doi: 10.3390/jcdd8080087

Figure 5.

Figure 5

Ectopic expression of Pcnt S impairs DNA synthesis in C2C12 myoblasts. (A) Representative examples of transfected C2C12 myoblast (GFP, green) labeled with the nucleotide analog EdU (red). Nuclei were visualized with DAPI (DNA). (B,D) Quantitative analysis of EdU+/GFP+ (B) and EdU+/GFP− (D) myoblasts upon ectopic expression of eGFP (control), Pcnt S- and Pcnt B-T2A-eGFP as indicated. (C,E) Bayesian posterior distribution for the data in (B,D). Red asterisk: GFP+ myoblast. Yellow asterisk: EDU+ myoblast. Scale bars: 50 µm (zoom) and 500 µm (overview). For the experiments, ≥274 myoblasts were analyzed per experimental condition. Data are mean ± SD, n = 3, n.s.: p > 0.05, ***: p < 0.001.