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. 2021 Aug 27;7(35):eabe3771. doi: 10.1126/sciadv.abe3771

Fig. 5. PADI4 is required for histone methylation and acetylation at HREs.

Fig. 5

(A to D) NTC and PADI4-knockdown (shP4#4 and shP4#5) subclones (A and B) or NTC and HIF-DKD subclones (C and D) were exposed to 20 or 1% O2 for 16 hours, and ChIP assays were performed using Ab against H3K4me3 (A and C) or H3K36me3 (B and D). Results were normalized to NTC at 20% O2 (mean ± SD; n = 3). #P < 0.05, ##P < 0.01, ###P < 0.001 versus NTC at 20% O2; *P < 0.05, **P < 0.01, ***P < 0.01 versus NTC at 1% O2 (two-way ANOVA). (E and F) SUM159 subclones were exposed to 20 or 1% O2, and ChIP assays were performed using Ab against H3K4ac (E) or H4K5ac (F). Results were normalized to NTC at 20% O2 (mean ± SD; n = 3). ***P < 0.001 versus NTC at 20% O2; ###P < 0.01 versus NTC at 1% O2 (two-way ANOVA).