FIG. 3.

Replacement of ETH1. MKP-o (wild type), DRY373 (apn1), RBY31 (eth1), and RBY71 (apn1 eth1) contain a yeast expression vector, pYES2 (top four strains in each panel), or the ETH1 expression vector, pETH1 (bottom four strains in each panel). The growth medium used for the gradient plate is CM−ura+gal (selection for the plasmid and inducing conditions [A to D]) or CM−ura+glu (selection for the plasmid and noninducing conditions [E to H]). The bottom layer does not contain MMS (A and E) or contains 0.00313% MMS (B and F), 0.00625% MMS (C and G), or 0.025% MMS (D and H).