Table 1. Nicked Loop Aminoacyl Transfer of tRNA Acceptor Arm Mimicsa.
aminoacyl
donor RNA strand |
||||||
---|---|---|---|---|---|---|
5′-l-Ala-pAGCGA |
5′-d-Ala-pAGCGA |
5′-Gly-pAGCGA |
||||
acceptor RNA strand | observed yield | corrected yield | observed yield | corrected yield | observed yield | corrected yield |
5′-UCGCUUGCCA-3′ | 30% | 55% | 10% | 15% | 9% | 11% |
5′-UCGCUUACCA-3′ | 16% | 30% | N.D. | N.D. | N.D. | N.D. |
5′-UCGCUUCCCA-3′ | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
5′-UCGCUUUCCA-3′ | 34% | 57% | 4% | 6% | 2% | 3% |
5′-UCGCUUGCCC-3′ | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
5′-UCGCUUGCCG-3′ | 14% | 25% | 8% | 12% | N.D. | N.D. |
5′-UCGCUUGCCU-3′ | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
5′-UCGCUUGCCA(2′d)-3′ | 4% | 6% | – | – | – | – |
5′-UCGCUUGCCA(3′d)-3′ | N.D. | N.D. | – | – | – | – |
5′-UCGCUUGCCCA-3′ | 22% | 38% | 15% | 25% | 17% | 23% |
5′-UCGCUACCA-3′ | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
5′-UCGCUUCCA-3′ | 1% | 2% | 2% | 4% | N.D. | N.D. |
N.D.: product not detected. −:, experiments not performed.