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. 2021 Jul 20;143(30):11836–11842. doi: 10.1021/jacs.1c05746

Table 2. Nicked Loop N-Acetylaminoacyl Transfer of tRNA Acceptor Arm Mimicsa.

graphic file with name ja1c05746_0005.jpg

  N-acetylaminoacyl donor RNA strand
  5′-Ac-l/d-Ala-pAGCGA
5′-Ac-Gly-pAGCGA
acceptor RNA strand observed yield corrected yield observed yield corrected yield
5′-UCGCUUGCCA-3′ 20% 25% 32% 46%
5′-UCGCUUACCA-3′ 9% 10% 27% 36%
5′-UCGCUUCCCA-3′ 8% 9% 36% 46%
5′-UCGCUUUCCA-3′ 14% 16% 21% 28%
5′-UCGCUUGCCC-3′ N.D. N.D. 5% 7%
5′-UCGCUUGCCG-3′ 12% 13% 25% 36%
5′-UCGCUUGCCU-3′ N.D. N.D. 5% 7%
5′-UCGCUUGCCA(2′d)-3′ 3% 4% 17% 23%
5′-UCGCUUGCCA(3′d)-3′ 2% 2% 14% 19%
5′-UCGCUUGCCCA-3′ 11% 14% 20% 32%
5′-UCGCUACCA-3′ 10% 13% 23% 37%
5′-UCGCUUCCA-3′ 2% 3% 4% 6%
a

N.D.: product not detected.