Table 2. Nicked Loop N-Acetylaminoacyl Transfer of tRNA Acceptor Arm Mimicsa.
N-acetylaminoacyl donor RNA strand |
||||
---|---|---|---|---|
5′-Ac-l/d-Ala-pAGCGA |
5′-Ac-Gly-pAGCGA |
|||
acceptor RNA strand | observed yield | corrected yield | observed yield | corrected yield |
5′-UCGCUUGCCA-3′ | 20% | 25% | 32% | 46% |
5′-UCGCUUACCA-3′ | 9% | 10% | 27% | 36% |
5′-UCGCUUCCCA-3′ | 8% | 9% | 36% | 46% |
5′-UCGCUUUCCA-3′ | 14% | 16% | 21% | 28% |
5′-UCGCUUGCCC-3′ | N.D. | N.D. | 5% | 7% |
5′-UCGCUUGCCG-3′ | 12% | 13% | 25% | 36% |
5′-UCGCUUGCCU-3′ | N.D. | N.D. | 5% | 7% |
5′-UCGCUUGCCA(2′d)-3′ | 3% | 4% | 17% | 23% |
5′-UCGCUUGCCA(3′d)-3′ | 2% | 2% | 14% | 19% |
5′-UCGCUUGCCCA-3′ | 11% | 14% | 20% | 32% |
5′-UCGCUACCA-3′ | 10% | 13% | 23% | 37% |
5′-UCGCUUCCA-3′ | 2% | 3% | 4% | 6% |
N.D.: product not detected.