Figure 2.

MFN2-silenced macrophages show increased pro-inflammatory responses to zymosan. (A) mRNA expression of indicated genes in macrophages transfected with control and MFN2 siRNAs and treated with 50 µg/ml zymosan for 3 h (B) TNFα, IL-6, and IL-1β secretion to supernatants of control and MFN2-silenced macrophages. Macrophages were incubated with 50 µg/ml zymosan for 24 h prior to analysis. (C) Western blot analysis and quantification of cyclooxygenase-2 (COX2) protein in control and MFN2-silenced macrophages after 24 h incubation with 50 µg/ml zymosan. (D) Prostaglandin E₂ (PGE₂) release into culture medium of control and MFN2-silenced macrophages after 24 h treatment with 50 µg/ml zymosan. All results are shown as means ± SEM of at least three independent experiments compared using one-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test or Student unpaired, two-tailed t-test; *p < 0.05; **p < 0.01; ***p < 0.001.