Skip to main content
. 2021 Aug 12;12:723683. doi: 10.3389/fimmu.2021.723683

Figure 3.

Figure 3

MFN2 silencing enhances NF‐κB and MAPK signal transduction pathways in zymosan-treated macrophages. (A) Western blot analysis of IκB, IKKβ, and JNK phosphorylation in macrophages transfected with control and MFN2 siRNAs and treated with 50 µg/ml zymosan for 0.5 or 1 h Quantification is shown only for 1 h (B) Western blot analysis and quantification of ERK, Syk, and Src phosphorylation in macrophages transfected with control and MFN2 siRNAs and stimulated with 50 µg/ml zymosan for 30 min. (C) Western blot analysis and quantification of IKKβ and JNK phosphorylation in macrophages pre-incubated for 30 min with Src inhibitor (Src I, 5 µM) followed by zymosan (50 µg/ml) stimulation for 30 min. (D) Median fluorescence intensity (MFI) of macrophages transfected with control or MFN2 siRNAs following phagocytosis of Alexa Fluor 594-labeled zymosan particles (10 µg/ml) for 30 or 60 min. (E) Total cellular ROS in zymosan-treated (50 µg/ml, 30 min) siCont or siMFN2-transfected macrophages measured using Amplex Red. All results are shown as means ± SEM of at least three independent experiments compared using one-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test or Student unpaired, two-tailed t-test; *p < 0.05; ***p < 0.001; ns, not significant.