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. 2021 Aug 12;12:723683. doi: 10.3389/fimmu.2021.723683

Figure 4.

Figure 4

MFN2 silencing increases inflammatory responses in macrophages treated with TLR2 and TLR4 agonists. (A) mRNA expression of indicated genes in macrophages transfected with control and MFN2 siRNAs and treated with 10 ng/ml Pam2CSK4 (PAM2) for 3 h (B) Western blot analysis of IKKβ and p38 phosphorylation in macrophages transfected with control and MFN2 siRNAs and stimulated with 10 ng/ml PAM2 for 0.5 and 1 h Quantification is shown only for 0.5 h (C) mRNA expression of indicated genes in macrophages transfected with control and MFN2 siRNAs and treated with 100 ng/ml lipopolysaccharide (LPS) for 3 h (D) Western blot analysis and quantification of IKKβ phosphorylation in macrophages transfected with control and MFN2 siRNAs and stimulated with 100 ng/ml lipopolysaccharide (LPS) for 30 min. All results are shown as means ± SEM of at least three independent experiments compared using one-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test; *p < 0.05; **p < 0.01; ****p < 0.0001; ns, not significant.