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. 2021 Aug 12;12:711902. doi: 10.3389/fendo.2021.711902

Figure 2.

Figure 2

Morphology and identification of GCs and therapeutic effects of hUCMSC-Exos in CTX-damaged GCs. (A) Morphologies of mouse ovarian GCs after isolation at 48 h and 72 h. Scale bar: 200 μm. (B) Identification of GCs by FSHR immunofluorescence. Scale bar: 100 μm. (C) EdU assay showed hUCMSC-Exos promoted the proliferative of GCs; cell nuclei were stained blue, while cells with proliferation activity were stained red. Scale bar: 50 μm. (D) Proliferation ratio of GCs was analyzed through the EdU assay. (E) Effects of hUCMSC-Exos on GCs viability were assessed by the CCK-8 assay from day 0 to day 5. (F) Representative immunoblots of PCNA and FSHR, using GAPDH as the reference. Quantification of protein expression revealed that exosomal treatment increased the expression levels of (G) PCNA and (H) FSHR. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ns, no significance. Data are representative of three independent experiments in each group.