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. 2021 Aug 12;9:681790. doi: 10.3389/fcell.2021.681790

FIGURE 2.

FIGURE 2

Administration of M5Ab attenuates the expression of OPN-N in ApoE−/− mice with AAA. (A) ELISA analyses of the levels of serum OPN-N in mice under different treatments. (B) Western blotting for OPN-N in mice under different treatments (left), bar graph showing quantification of OPN-N protein expression (right). β-actin was used as an internal control. (C,D) Representative images of aortic immunohistochemistry staining to detect OPN-N expression in mice (black arrows), quantification of OPN-N area. Scar bar 200 μm, boxed areas; Scar bar 50 μm, main images. (E,F) OPN-N immunofluorescence staining of aortic tissues in mice and quantification of OPN-N intensity (Scar bar 50 μm), boxed areas in F are shown at higher magnification below (Scar bar 20 μm). Data are expressed as means ± SEM; n = 3 per group. ∗P < 0.05 vs the Ang II + M5Ab group; ∗∗P < 0.05 vs the control and control + M5Ab groups. Abbreviations: Ang II, angiotensin II; OPN-N, osteopontin N-terminal fragment. DAPI staining of nuclei: blue; OPN-N staining: red. Ta, indicates tunica adventitia; Int, intima; Med, media; L, lumen; and IT, intraluminal thrombus.